ap2α staining Search Results


anti ap2α antibody  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank anti ap2α antibody
A–C . Retinal sections of CTL and STZ-treated P6 rat pups were stained with various antibodies specific to neurons of the INL (A), photoreceptors cells (B) and glial cells (C) of the retina. A : INL neuron generation was not affected by hyperglycemia and similar patterns of staining (red) were observed in CTL and STZ animals for PKCα (bipolar cells), <t>Ap2α</t> (amacrine cells) and calretinin (CALR, horizontal cells). B . Photoreceptor generation was not affected by hyperglycemia and similar patterns of staining (red) were observed for peanut agglutinin (PNA, cones), Rho4D2 (R4D2, rods) in STZ P6 animals compared to CTL. C . Muller cells cell genesis was not affected by hyperglycemia when compared to CTL. Nuclei were counterstained with DAPI. Scale bars 50 µm. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer.
Anti Ap2α Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap2%CE%B1+staining/anti-AP-2+alpha/pmc03836846-43-22-33
Average 96 stars, based on 1 article reviews
anti ap2α antibody - by Bioz Stars, 2026-10
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ap2α  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology ap2α
Underexpressed and overexpressed genes in pancreatic ductal adenocarcinoma with lymph node metastasis identified by expression profile
Ap2α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap2%CE%B1+staining/AP-2%CE%B1+Antibody/pmc11159850-197-18-22
Average 95 stars, based on 1 article reviews
ap2α - by Bioz Stars, 2026-10
95/100 stars
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ap2α  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc ap2α
a Coomassie blue staining for HA pulldown of EZH2 complex in RPMI8226 cells transfected with HA-EZH2 plasmid. The proteins identified are indicated on the right. b Co-immunoprecipitation of EZH2 or SUZ12 with <t>AP2α</t> in HEK293T cells. c Pull-down of HA-EZH2 or HA-SUZ12 with AP2α in HEK293T cells. d Co-immunoprecipitation of EZH2 or AP2α in myeloma cells. e Immunofluorescent staining of myeloma cells with DAPI and antibodies against EZH2 and AP2α. Scale bar, 2 μm. f Schematic of the truncations including ΔAct, ΔBas, and ΔDim fragments. g Western blotting shows truncations of AP2α in HEK293T cells. h Pull-down of Myc-EZH2 with truncations of HA-AP2α in HEK293T cells. i Immunofluorescent staining of patient samples with DAPI and antibodies against CD138, EZH2, or AP2α (n = 5). Scale bar, 10 μm. j Schematic of the EMP1 promoter luciferase reporter. Solid boxes: promoter region; red crosses: mutations site. k The luciferase assay of EMP1 promoter. ns, not significant. l ChIP assay shows AP2α enrichment in EMP1 promoter in patient cells (left panel) (n = 5) or cell lines (right panel) (n = 5 biological replicates). m Western blotting shows the expression of AP2α in cells transfected with AP2α shRNAs and non-target control (sh Ctrl ). The samples derive from the same experiment but different gels for each protein and processed in parallel ( b – d , g , h , m ). n , o ChIP assay shows enrichment of SUZ12, EZH2, and H3K27me3 in EMP1 gene promoter in sh Ctrl or sh AP2α transfected Pt MM (n = 5), RPMI8226 or MM.1S cells. p Formation of TRAP + cells and Alizarin red S staining for osteoblast differentiation, cultured with sh Ctrl or sh AP2α myeloma cells. q Schematic of the recruitment of PRC2 complex by AP2α to the EMP1 promoter. a – e , g , h , k , m , o , p are representative of three independent experiments. Data are means ± SD. k , l : p values were determined by unpaired two-tailed t test; n – p : p values were determined using one-way ANOVA with Tukey’s test. Source data are provided as a Source data file.
Ap2α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap2%CE%B1+staining/AP-2alpha+Rabbit+mAb/pmc11782520-367-15-17
Average 93 stars, based on 1 article reviews
ap2α - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


A–C . Retinal sections of CTL and STZ-treated P6 rat pups were stained with various antibodies specific to neurons of the INL (A), photoreceptors cells (B) and glial cells (C) of the retina. A : INL neuron generation was not affected by hyperglycemia and similar patterns of staining (red) were observed in CTL and STZ animals for PKCα (bipolar cells), Ap2α (amacrine cells) and calretinin (CALR, horizontal cells). B . Photoreceptor generation was not affected by hyperglycemia and similar patterns of staining (red) were observed for peanut agglutinin (PNA, cones), Rho4D2 (R4D2, rods) in STZ P6 animals compared to CTL. C . Muller cells cell genesis was not affected by hyperglycemia when compared to CTL. Nuclei were counterstained with DAPI. Scale bars 50 µm. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer.

Journal: PLoS ONE

Article Title: Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina

doi: 10.1371/journal.pone.0079545

Figure Lengend Snippet: A–C . Retinal sections of CTL and STZ-treated P6 rat pups were stained with various antibodies specific to neurons of the INL (A), photoreceptors cells (B) and glial cells (C) of the retina. A : INL neuron generation was not affected by hyperglycemia and similar patterns of staining (red) were observed in CTL and STZ animals for PKCα (bipolar cells), Ap2α (amacrine cells) and calretinin (CALR, horizontal cells). B . Photoreceptor generation was not affected by hyperglycemia and similar patterns of staining (red) were observed for peanut agglutinin (PNA, cones), Rho4D2 (R4D2, rods) in STZ P6 animals compared to CTL. C . Muller cells cell genesis was not affected by hyperglycemia when compared to CTL. Nuclei were counterstained with DAPI. Scale bars 50 µm. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer.

Article Snippet: In addition to the antibodies used on retinal flatmounts, the following primary antibodies were used to study retinal cells type on cryosections: anti-Ap2α antibody (3B5, for amacrine and horizontal cells) was purchased to Developmental Studies Hybridoma Bank (DHSB, Iowa city, Iowa); mouse anti-glutamine synthetase antibody (MAB302, for Muller cells) was obtained from Millipore (Saint-Quentin Fallavier, France), and rabbit anti-protein kinase Cα antibody (PKCα) (Sc-208, for bipolar cells) from Santa Cruz (Heidelberg, Germany).

Techniques: Staining

Underexpressed and overexpressed genes in pancreatic ductal adenocarcinoma with lymph node metastasis identified by expression profile

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Underexpressed and overexpressed genes in pancreatic ductal adenocarcinoma with lymph node metastasis identified by expression profile

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Expressing, Binding Assay, Membrane, Sequencing

Immunohistochemical analysis between pancreatic ductal adenocarcinoma patients with and without lymph node metastasis

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Immunohistochemical analysis between pancreatic ductal adenocarcinoma patients with and without lymph node metastasis

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Immunohistochemical staining, Biomarker Discovery

Immunohistochemical staining of four genes associated with lymph node metastasis in pancreatic ductal adenocarcinoma patients (A–D). Activating enhancer binding protein 2 (AP2α) expressed in nucleus of pancreatic cancer cells (A). Liver–intestine cadherin (LI‐cadherin) (B), mucin 17 (MUC17) (C), and X‐linked Kx blood group (XK) (D) expressed in membrane of pancreatic cancer cells.

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Immunohistochemical staining of four genes associated with lymph node metastasis in pancreatic ductal adenocarcinoma patients (A–D). Activating enhancer binding protein 2 (AP2α) expressed in nucleus of pancreatic cancer cells (A). Liver–intestine cadherin (LI‐cadherin) (B), mucin 17 (MUC17) (C), and X‐linked Kx blood group (XK) (D) expressed in membrane of pancreatic cancer cells.

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Immunohistochemical staining, Staining, Binding Assay, Membrane

Univariate and multivariate analysis of factors associated with lymph node metastasis in pancreatic ductal adenocarcinoma ( n = 63)

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Univariate and multivariate analysis of factors associated with lymph node metastasis in pancreatic ductal adenocarcinoma ( n = 63)

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Expressing

(A) Overall survival (OS) without lymph node metastasis was better (median, 844 vs 470 days). (B) OS with AP2α‐low expression was worse than that of high expression. (C) OS with MUC17‐high expression was worse than that of low expression (median, 451 vs 567 days). (D) OS with AP2α‐low and MUC17‐high expression was worse than that of AP2α‐high and MUC17‐low expression. LNM (+), positive lymph node metastasis; LNM (−), negative lymph node metastasis.

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: (A) Overall survival (OS) without lymph node metastasis was better (median, 844 vs 470 days). (B) OS with AP2α‐low expression was worse than that of high expression. (C) OS with MUC17‐high expression was worse than that of low expression (median, 451 vs 567 days). (D) OS with AP2α‐low and MUC17‐high expression was worse than that of AP2α‐high and MUC17‐low expression. LNM (+), positive lymph node metastasis; LNM (−), negative lymph node metastasis.

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Expressing

Multivariate analysis using Cox’s proportional hazards regression model to determine prognostic parameters in patients with pancreatic ductal adenocarcinoma ( n = 63)

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Multivariate analysis using Cox’s proportional hazards regression model to determine prognostic parameters in patients with pancreatic ductal adenocarcinoma ( n = 63)

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Expressing

Accuracy for lymph node metastasis in pancreatic ductal adenocarcinoma by immunohistochemical staining intensities of 7 genes using all available cut‐off points in the training set ( n = 20)

Journal: Cancer Science

Article Title: Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome‐wide expression profiling

doi: 10.1111/j.1349-7006.2009.01359.x

Figure Lengend Snippet: Accuracy for lymph node metastasis in pancreatic ductal adenocarcinoma by immunohistochemical staining intensities of 7 genes using all available cut‐off points in the training set ( n = 20)

Article Snippet: Primary antibodies diluted in PBS as follows: DOK7 (1:100, rabbit polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), AP2α (1:50, mouse monoclonal; Santa Cruz Biotechnology), FOXL1 (1:1000, rabbit polyclonal; CeMines, Evergreen, CO, USA), LI‐cadherin (1:150, goat polyclonal; Santa Cruz Biotechnology), Granzyme A (1:50, mouse monoclonal; Abcam, Cambridge, UK): MUC17 (1:150, goat polyclonal; Santa Cruz Biotechnology), C4BPB (1:25, goat polyclonal; Santa Cruz Biotechnology), XK (1:100, goat polyclonal; Santa Cruz Biotechnology), and LSD1 (1:1000, mouse monoclonal; Abcam).

Techniques: Immunohistochemical staining, Staining

a Coomassie blue staining for HA pulldown of EZH2 complex in RPMI8226 cells transfected with HA-EZH2 plasmid. The proteins identified are indicated on the right. b Co-immunoprecipitation of EZH2 or SUZ12 with AP2α in HEK293T cells. c Pull-down of HA-EZH2 or HA-SUZ12 with AP2α in HEK293T cells. d Co-immunoprecipitation of EZH2 or AP2α in myeloma cells. e Immunofluorescent staining of myeloma cells with DAPI and antibodies against EZH2 and AP2α. Scale bar, 2 μm. f Schematic of the truncations including ΔAct, ΔBas, and ΔDim fragments. g Western blotting shows truncations of AP2α in HEK293T cells. h Pull-down of Myc-EZH2 with truncations of HA-AP2α in HEK293T cells. i Immunofluorescent staining of patient samples with DAPI and antibodies against CD138, EZH2, or AP2α (n = 5). Scale bar, 10 μm. j Schematic of the EMP1 promoter luciferase reporter. Solid boxes: promoter region; red crosses: mutations site. k The luciferase assay of EMP1 promoter. ns, not significant. l ChIP assay shows AP2α enrichment in EMP1 promoter in patient cells (left panel) (n = 5) or cell lines (right panel) (n = 5 biological replicates). m Western blotting shows the expression of AP2α in cells transfected with AP2α shRNAs and non-target control (sh Ctrl ). The samples derive from the same experiment but different gels for each protein and processed in parallel ( b – d , g , h , m ). n , o ChIP assay shows enrichment of SUZ12, EZH2, and H3K27me3 in EMP1 gene promoter in sh Ctrl or sh AP2α transfected Pt MM (n = 5), RPMI8226 or MM.1S cells. p Formation of TRAP + cells and Alizarin red S staining for osteoblast differentiation, cultured with sh Ctrl or sh AP2α myeloma cells. q Schematic of the recruitment of PRC2 complex by AP2α to the EMP1 promoter. a – e , g , h , k , m , o , p are representative of three independent experiments. Data are means ± SD. k , l : p values were determined by unpaired two-tailed t test; n – p : p values were determined using one-way ANOVA with Tukey’s test. Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction

doi: 10.1038/s41467-025-56506-5

Figure Lengend Snippet: a Coomassie blue staining for HA pulldown of EZH2 complex in RPMI8226 cells transfected with HA-EZH2 plasmid. The proteins identified are indicated on the right. b Co-immunoprecipitation of EZH2 or SUZ12 with AP2α in HEK293T cells. c Pull-down of HA-EZH2 or HA-SUZ12 with AP2α in HEK293T cells. d Co-immunoprecipitation of EZH2 or AP2α in myeloma cells. e Immunofluorescent staining of myeloma cells with DAPI and antibodies against EZH2 and AP2α. Scale bar, 2 μm. f Schematic of the truncations including ΔAct, ΔBas, and ΔDim fragments. g Western blotting shows truncations of AP2α in HEK293T cells. h Pull-down of Myc-EZH2 with truncations of HA-AP2α in HEK293T cells. i Immunofluorescent staining of patient samples with DAPI and antibodies against CD138, EZH2, or AP2α (n = 5). Scale bar, 10 μm. j Schematic of the EMP1 promoter luciferase reporter. Solid boxes: promoter region; red crosses: mutations site. k The luciferase assay of EMP1 promoter. ns, not significant. l ChIP assay shows AP2α enrichment in EMP1 promoter in patient cells (left panel) (n = 5) or cell lines (right panel) (n = 5 biological replicates). m Western blotting shows the expression of AP2α in cells transfected with AP2α shRNAs and non-target control (sh Ctrl ). The samples derive from the same experiment but different gels for each protein and processed in parallel ( b – d , g , h , m ). n , o ChIP assay shows enrichment of SUZ12, EZH2, and H3K27me3 in EMP1 gene promoter in sh Ctrl or sh AP2α transfected Pt MM (n = 5), RPMI8226 or MM.1S cells. p Formation of TRAP + cells and Alizarin red S staining for osteoblast differentiation, cultured with sh Ctrl or sh AP2α myeloma cells. q Schematic of the recruitment of PRC2 complex by AP2α to the EMP1 promoter. a – e , g , h , k , m , o , p are representative of three independent experiments. Data are means ± SD. k , l : p values were determined by unpaired two-tailed t test; n – p : p values were determined using one-way ANOVA with Tukey’s test. Source data are provided as a Source data file.

Article Snippet: Slides were stained with anti-CD138 (#AF2780, R&D system, 1:100), EZH2 (#MA5-18108, Thermo Fisher Scientific, 1:25), AP2α (#3215, Cell Signaling Technology, 1:100) or EMP1 (#ab230445, Abcam, 1:50) overnight at 4 °C.

Techniques: Staining, Transfection, Plasmid Preparation, Immunoprecipitation, Western Blot, Luciferase, Expressing, Control, Cell Culture, Two Tailed Test